| Florida Alliance of Public Hospitals, Legislative Hero Award 2009 |
| Florida Association of Assisted Living Facilities, Legislator of the Year 2009 |
| Florida Association of Rehabilitative Facilities (A Voice for Community Agencies), Legislative Champion Award – "For the impact Rep Ambler's efforts have made in the lives of Florida citizens with disabilities – through his support, thousands of individuals with disabilities have achieved a better quality of life." 2009 |
| Florida Chiropractic Association, D.J. Rainey Legislative Award for Outstanding Contribution to Quality Health Care for the Citizens of Florida, 2009 |
| Florida Health Care Association, Legislator of the Year 2009 |
| Florida Hospital Association, Legislator of the Year 2009 |
| Florida Justice Association, Outstanding Representative Award – In recognition of extraordinary legislative leadership and dedicated service on behalf of the citizens of the State of Florida 2009 |
| Florida Land Title Association, Legislator of the Year 2009 – "Presented in honor and appreciation of his dedicated leadership and service in recognition of the critical role of real property to sustained job creation and economic growth benefiting all citizens of the State of Florida." |
| Florida Pharmacy Association, Legislator of the Year 2009 |
| Florida Safety Net Hospitals, Legislative Hero Award 2009 |
| American Association of School Boards, 2008 Magna Award, Most Innovative Education Program in America for "Ought To Be A Law" Student Legislation Program |
| Associated Builders and Contractors, Friend of Free Enterprise Award 2008 |
| Associated Industries of Florida Ports Council, Legislator of the Year 2008 |
| Christian Coalition of Florida, Faith & Family Award 2008 |
| The Florida Bar, Legislator of the Year 2008 |
| Florida Chamber of Commerce, Honor Roll 2004, 2005, 2006, 2007, 2008 |
| Florida Ports Council, Tug boat Award – Presented for support and commitment to Florida's Seaports, with respect and appreciation for efforts to ensure sustainability of Florida's industry and to promote economic development and job growth in Florida 2008 |
| Independent Colleges & Universities, ICUF All-Star Award – Presented for being "a tireless advocate for Florida students" 2008 |
| Friends of the Hillsborough County Regional Science and Engineering Fair, Award of Appreciation 2007 |
| Florida Academy of Trial Lawyers, Outstanding Representative Award – In recognition of extraordinary legislative leadership & dedicated service to the citizens of Florida 2006 |
| Florida Association of Area Agency on Aging, Certificate of Appreciation 2006 |
| Florida Bar Military Affairs Committee Award – In recognition and appreciation of the outstanding contribution to the Military Affairs Committee 2004, 2005, 2006 |
| Florida Association of Mortgage Brokers, West Coast Chapter, Tampa, Florida, Legislative Award in Recognition for Service to the Community 2005 |
| Public Safety Legislative Award 2004-2005 |
| Consumer Rights, Legislative Award 2004 |
| Moffitt Cancer Center, Outstanding Legislator Award 2004 |
| Brentwood Park Homeowners Association, Certificate of Appreciation for ten years of dedicated service as President 2002 |
| Hillsborough County, "Have A Heart" Award for Outstanding Pro Bono Attorney of the Year 2002 |
| Air Force Achievement Medal |
| Air Force Commendation Medal |
| Air Force Expert Marksmanship Ribbon |
| Air Force Longevity Ribbon |
| Air Force Meritorious Service Medal with Oak Leaf Cluster |
| Air Force Outstanding Unit Award |
| Air Force Recognition Ribbon (USAF Harmon Award for Top Reserve Judge Advocate) |
| National Defense Medal |
| "Right Stuff" Award – Outstanding Jr. Officer, 56th Combat Support Group |
| Tactical Air Command Judge Advocate of the Year |
